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ZFIN ID: ZDB-FIG-130318-13
Lu et al., 2013 - Failure in closure of the anterior neural tube causes left isomerization of the zebrafish epithalamus. Developmental Biology   374(2):333-344 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage Range: 5-9 somites to 10-13 somites

Fig. 4 The neural crest gene crestin is expressed in embryos with an open neural tube. sqt embryos with an open neural tube were identified by two-color WISH by first detecting flh in the pineal with a red precipitate (not shown as it is out of the focal plane of the image). The crestin signal in the neural crest cells was then detected via a purple precipitate. (A) In WT embryos, crestin is expressed in a large region next to rhombomeres 4–6 (open arrowheads), and in two stripes of cells on the left right sides at the borders of the folding neuroepithelium. ((B) and (C)) In sqt embryos with an open neural tube, both regions of crestin are present, although they are further apart. The experiment was repeated three times and representative images are shown. Embryos were fixed at the 8–10 somite stage. All images are dorsal views with anterior to the left. Scale bar=50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
crestin WT standard conditions 5-9 somites to 10-13 somites neural crest cell ISH
ndr1cz35/cz35 standard conditions 5-9 somites to 10-13 somites neural crest cell ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 374(2), Lu, P.N., Lund, C., Khuansuwan, S., Schumann, A., Harney-Tolo, M., Gamse, J.T., and Liang, J.O., Failure in closure of the anterior neural tube causes left isomerization of the zebrafish epithalamus, 333-344, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.