Fig. 4
- ID
- ZDB-FIG-130318-13
- Publication
- Lu et al., 2013 - Failure in closure of the anterior neural tube causes left isomerization of the zebrafish epithalamus
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The neural crest gene crestin is expressed in embryos with an open neural tube. sqt embryos with an open neural tube were identified by two-color WISH by first detecting flh in the pineal with a red precipitate (not shown as it is out of the focal plane of the image). The crestin signal in the neural crest cells was then detected via a purple precipitate. (A) In WT embryos, crestin is expressed in a large region next to rhombomeres 4–6 (open arrowheads), and in two stripes of cells on the left right sides at the borders of the folding neuroepithelium. ((B) and (C)) In sqt embryos with an open neural tube, both regions of crestin are present, although they are further apart. The experiment was repeated three times and representative images are shown. Embryos were fixed at the 8–10 somite stage. All images are dorsal views with anterior to the left. Scale bar=50 μm. |
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| Stage Range: | 5-9 somites to 10-13 somites |
Reprinted from Developmental Biology, 374(2), Lu, P.N., Lund, C., Khuansuwan, S., Schumann, A., Harney-Tolo, M., Gamse, J.T., and Liang, J.O., Failure in closure of the anterior neural tube causes left isomerization of the zebrafish epithalamus, 333-344, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.