FIGURE

Fig. 1

ID

ZDB-FIG-130304-17

Publication

Bohnsack et al., 2013 - Thyroid hormone and retinoic acid interact to regulate zebrafish craniofacial neural crest development

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Fig. 1

TH regulates early neural crest development. Thraa MO knockdown (decreased TH signaling) inhibited neural crest migration at 24 (B) and 48 hpf (E) vs. control (A, D) in Tg(foxd3::EGFP). Thraa MO knockdown inhibited expression at 24 hpf of crestin in the head (H) and body (K; between somites, arrows) vs. control (G, J). Dio3 MO knockdown (increased TH) in Tg(foxd3::EGFP) embryos inhibited PA (arrowheads) formation at 24 (C vs. A) and 48 hpf (F vs. D). In situ hybridization for crestin demonstrated Dio3 MO knockdown impaired neural crest migration in the head (I) and between somites (L, arrows) at 24 hpf. Asterisk denotes developing eye. Scale bar=50 or 100 µm as indicated.

Expression Data

Genes:	crestin GFP
Fish:	WT WT + MO1-dio3b WT + MO2-thraa mpv17^a9; zf15Tg mpv17^a9; zf15Tg + MO1-dio3b mpv17^a9; zf15Tg + MO2-thraa
Condition:	chemical treatment: N-phenylthiourea
Knockdown Reagents:	MO1-dio3b MO2-thraa
Anatomical Terms:	epiphysis head neural crest neural crest trunk neural crest
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-dio3b mpv17^a9; zf15Tg + MO1-dio3b mpv17^a9; zf15Tg + MO2-thraa
Condition:	chemical treatment: N-phenylthiourea
Knockdown Reagents:	MO1-dio3b MO2-thraa
Observed In:	neural crest cell migration pharyngeal arch
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 373(2), Bohnsack, B.L., and Kahana, A., Thyroid hormone and retinoic acid interact to regulate zebrafish craniofacial neural crest development, 300-309, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.