FIGURE

Fig. 5

ID

ZDB-FIG-130228-2

Publication

Cerdà et al., 2002 - Molecular characterization of calymmin, a novel notochord sheath-associated extracellular matrix protein in the zebrafish embryo

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Fig. 5

Detection of Calymmin in wild-type and ntl mutant zebrafish at 22 hours postfertilization embryos. A,B: General views of wild-type (A) and ntl mutant (B) embryos (rostral to the left) after whole-mount immunocytochemistry. Note the absence of the notochord and tail in the ntl mutants, in which Calymmin was not detected. C: Coomassie brilliant blue-stained sodium dodecyl sulphate-polyacrylamide gel electrophoresis of cytoskeletal fractions from wild-type (lane 1) and ntl embryos (lane 2), and the corresponding immunoblot (lanes 1′ and 2′), showing the absence of the anti-Calymmin reactive band in the mutant embryos. The relative molecular masses of the reference proteins are indicated on the left.

Expression Data

Gene:	cmn
Antibody:	Ab1-cmn
Fish:	WT tbxta^tc41/tc41
Anatomical Terms:	notochord whole organism
Stage:	26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tbxta^tc41/tc41
Observed In:	notochord formation notochord posterior region post-anal tail morphogenesis post-vent region
Stage:	26+ somites

Phenotype Detail

Acknowledgments

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