|
Fig. 5
Detection of Calymmin in wild-type and ntl mutant zebrafish at 22 hours postfertilization embryos. A,B: General views of wild-type (A) and ntl mutant (B) embryos (rostral to the left) after whole-mount immunocytochemistry. Note the absence of the notochord and tail in the ntl mutants, in which Calymmin was not detected. C: Coomassie brilliant blue-stained sodium dodecyl sulphate-polyacrylamide gel electrophoresis of cytoskeletal fractions from wild-type (lane 1) and ntl embryos (lane 2), and the corresponding immunoblot (lanes 1′ and 2′), showing the absence of the anti-Calymmin reactive band in the mutant embryos. The relative molecular masses of the reference proteins are indicated on the left.