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ZFIN ID: ZDB-FIG-130220-6
Ding et al., 2013 - Redundant roles of PRDM family members in zebrafish craniofacial development. Developmental dynamics : an official publication of the American Association of Anatomists   242(1):67-79 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-15 to Pec-fin

Fig. 1 Expression of different prdms in developing zebrafish embryos. In situ hybridization (ISH) using prdm3, 5, and 16 at 30-hpf (A, E, I), 48-hpf (B, F, J), and 60-hpf stages showing lateral (C, G, K) and (D, H, L) ventral views. Note the partially overlapping expression of prdm3 and prdm16. A: Lateral view of whole mount embryo showing the prdm3 expression in the tegmentum, ventral diencephalon, neurocranium, hindbrain, pharyngeal arches at 30 hpf. At 48 hpf (B) and 60 hpf (C, D), prdm3 gradually increases and is highly expressed in pharyngeal arches, and is also expressed in pectoral fin buds, neurocranium, hindbrain, and tegmentum. E–H: prdm5 is not specifically expressed at 30 hpf, but is expressed at a low level in the area behind the eye and neurocranium at 48 and 60 hpf. The arrow in H points to the forming stomodeum in which prdm5 is expressed. I,J: From 30 to 48 hpf, prdm16 expression gradually increases in pharyngeal arches, as well as in neurocranium, pectoral fin buds, hindbrain, and olfactory placode. K,L: At 60 hpf, prdm16 expression decreases in the pharyngeal arches and hindbrain, but is still expressed at a significant level. Anterior is to the left in all panels. e, eye; fb, fin buds; hb, hindbrain; n, neurocranium; op, olfactory placode; pa, pharyngeal arch; tg, tegmentum; vd, ventral diencephalon.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mecom WT standard conditions Prim-15 diencephalon ventral region ISH
Prim-15 hindbrain ISH
Prim-15 pectoral fin bud ISH
Prim-15 pharyngeal arch ISH
Prim-15 tegmentum ISH
Long-pec neurocranium ISH
Long-pec pectoral fin ISH
Long-pec pharyngeal arch ISH
Pec-fin neurocranium ISH
Pec-fin pectoral fin ISH
Pec-fin pharyngeal arch ISH
prdm5 WT standard conditions Prim-15 hindbrain ISH
Prim-15 peripheral olfactory organ ISH
Prim-15 pharyngeal arch ISH
Long-pec hindbrain ISH
Long-pec neurocranium ISH
Long-pec pectoral fin ISH
Long-pec pharyngeal arch ISH
Pec-fin hindbrain ISH
Pec-fin neurocranium ISH
Pec-fin pectoral fin ISH
Pec-fin pharyngeal arch ISH
prdm16 WT standard conditions Prim-15 whole organism ISH
Long-pec neurocranium ISH
Pec-fin neurocranium ISH
Pec-fin stomodeum ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.