FIGURE

Fig. 3

ID

ZDB-FIG-130220-13

Publication

Luo et al., 2012 - Midkine-A functions upstream of Id2a to regulate cell cycle kinetics in the developing vertebrate retina

Other Figures

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Fig. 3

Mdka loss-of-function alters cell cycle kinetics. (A) BrdU labeled progenitors in uninjected (a) control (b) and Mdka loss-of-function embryos (c). (B) Histograms showing the average proportion of the retinal section occupied by BrdU-labeled cells. *P <0.001. n = 9 retinas/group. Scale bar equals 50μm.(C) Retinas of embryos at 48 hpf stained with antibodies against phosphohistone H3 (pH3) in uninjected (a), control (b), and Mdka loss-of-function embryos (c). (D) The number of pH3-stained cells/unit area. ^**P <0.01. n=12 embryos/group. MM, embryos injected with 5-pair mismatch control morpholinos; MO, embryos injected with mdka-targeted morpholinos (MO); UI, uninjected embryos. gcl, ganglion cell layer; inl, inner nuclear layer; L, lens; ON, optic nerve; onl, outer nuclear layer. Scale bar equals 50 μm. (E) Graph of the least-squares regression lines through data showing the proportion of BrdU-labeled progenitors as a function of time. An F-test was used to compare the control and experimental data. **P <0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-mdka
Knockdown Reagent:	MO1-mdka
Observed In:	retina cell cycle process retina cell population proliferation retina mitotic cell cycle retina S phase
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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