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ZFIN ID: ZDB-FIG-130208-7
Kizil et al., 2012 - Regenerative Neurogenesis from Neural Progenitor Cells Requires Injury-Induced Expression of Gata3. Developmental Cell   23(6):1230-1237 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage Range: Prim-5 to Adult

Fig. 4 Fgf Signaling Is Required for Injury-Induced Gata3 Expression(A) Heat-shock scheme.(B) Gata3 IHC on 3 dpl heat-shocked sham control fish telencephalon.(C) Gata3 IHC on 3 dpl heat-shocked Tg(hsp:dnfgfr1-gfp) fish telencephalon. Inset: double IHC for GFP and Gata3, showing uniform dnFgfr1-GFP.(D) High magnification from dorsomedial region of (B) with DAPI.(E) High magnification from dorsomedial region of (C) with DAPI.(F) gata3 ISH in heat-shocked control telencephalon at 3 dpl.(G) gata3 ISH in heat-shocked Tg(hsp:dnfgfr1-gfp) telencephalon at 3 dpl. Scale bars 20 μm. n = 4 fish for (B), 6 for (C), 6 for (D and E), and 5 for (F and G). Data are represented as mean ± SEM.(H) Heat-shock and amputation scheme for Tg(hsp70l:dnfgfr1) fish. (H′) gata3 ISH on 2 dpa nontransgenic siblings. (H′′) gata3 ISH on 2 dpa Tg(hsp70:dnfgfr1) fish.(I) Transient and brief heat-shock and amputation scheme for Tg(hsp70l:dnfgfr1) fish. (I2) Gata3 and GFP immunostaining on nontransgenic siblings shown for single fin ray. Dashed lines, amputation margin. (I′1–I′3) Individual fluorescence channel for Gata3, DAPI, and merged image. (I′′) Gata3 and GFP immunostaining on Tg(hsp:dnfgfr1-egfp) animals shown for single fin ray. Dashed lines, amputation margin.(J) spry4 expression at 24 hr postfertilization (hpf) embryo.(K) Blocking Fgf signaling abrogates spry4 expression.(L) gata3 expression at 24 hpf.(M) Blocking Fgf signaling does not alter the expression of gata3. (J′–M′) Dorsal view of animals in (J, K, L, and M), respectively. n = 5 for (H–H′′), 3 for (I–I′′), and more than 50 embryos for (J–M2). Scale bars 200 μm.See also Figure S4.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP pd1Tg physical alteration: anatomical structure, heat shock Adult telencephalon IHC
gata3 WT standard conditions Prim-5 anatomical structure ISH
Adult Not Detected telencephalon ISH
WT physical alteration: anatomical structure Adult caudal fin regenerating fin ISH
Adult telencephalon regenerating tissue ISH
pd1Tg heat shock Prim-5 anatomical structure ISH
pd1Tg physical alteration: anatomical structure, heat shock Adult Not Detected telencephalon ISH
spry4 WT standard conditions Prim-5 brain ISH
Prim-5 caudal fin ISH
pd1Tg heat shock Prim-5 Not Detected brain ISH
Prim-5 Not Detected caudal fin ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 23(6), Kizil, C., Kyritsis, N., Dudczig, S., Kroehne, V., Freudenreich, D., Kaslin, J., and Brand, M., Regenerative Neurogenesis from Neural Progenitor Cells Requires Injury-Induced Expression of Gata3, 1230-1237, Copyright (2012) with permission from Elsevier. Full text @ Dev. Cell