FIGURE

Fig. 5

ID

ZDB-FIG-130129-5

Publication

Luo et al., 2012 - Evidence of a role of inositol polyphosphate 5-phosphatase INPP5E in cilia formation in zebrafish

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Fig. 5

Pronephric cilia defect in Inpp5e morphants. (A) INPP5E mRNA rescue of Inpp5e pronephric cilia formation. Representative image of pronephric cilia of zebrafish embryos at 24 hpf stage, injected with control MO (4 ng), Inpp5e MO (4 ng) or Inpp5e MO (4 ng) and hINPP5E mRNA (500 ng), immunostaining with acetylated α-tubulin (red). Scale bar 10 µm. (B) Pronephric cilia length of control and Inpp5e MO. Pronephric cilia of zebrafish embryos injected with control MO (4 ng), Inpp5e MO (4 ng) or Inpp5e MO (4 ng) and hINPP5E mRNA (500 ng) at 24 hpf stage were analyzed by immunostaining with acetylated α-tubulin and cilia length was measured (N = 20 embryos, three independent experiments, unpaired t-test, ^* p = 8.57E08).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TU + MO1-inpp5e
Knockdown Reagent:	MO1-inpp5e
Observed In:	pronephric duct cilium
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Vision Research, 75, Luo, N., Lu, J., and Sun, Y., Evidence of a role of inositol polyphosphate 5-phosphatase INPP5E in cilia formation in zebrafish, 98-107, Copyright (2012) with permission from Elsevier. Full text @ Vision Res.