FIGURE

Fig. 4

ID

ZDB-FIG-130129-4

Publication

Luo et al., 2012 - Evidence of a role of inositol polyphosphate 5-phosphatase INPP5E in cilia formation in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Kupffer’s vesicle cilia defect in Inpp5e morphants. (A) Human INPP5E mRNA could rescue the loss of Inpp5e. KV cilia of zebrafish embryos injected with control MO (4 ng), Inpp5e MO (4 ng) or Inpp5e MO (4 ng) and hINPP5E mRNA (500 ng) at 6-somite stage were immunostained with acetylated α-tubulin (red), representative images are shown (dash line indicates border of KV). (B and C) Quantification of number (B) and length (C) of KV cilia in zebrafish embryos injected with control MO (4 ng), Inpp5e MO (4 ng) or Inpp5e MO (4 ng) and hINPP5E mRNA (500 ng) (N = 20 embryos, three independent experiments, unpaired t-test, ^* p = 0.02 in (B) and ^* p = 1.56E08 in (C)).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TU + MO1-inpp5e
Knockdown Reagent:	MO1-inpp5e
Observed In:	Kupffer's vesicle cilium
Stage:	5-9 somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Vision Research, 75, Luo, N., Lu, J., and Sun, Y., Evidence of a role of inositol polyphosphate 5-phosphatase INPP5E in cilia formation in zebrafish, 98-107, Copyright (2012) with permission from Elsevier. Full text @ Vision Res.