FIGURE

Fig. 2

ID

ZDB-FIG-130129-18

Publication

Chu et al., 2013 - A zebrafish model of congenital disorders of glycosylation with phosphomannose isomerase deficiency reveals an early opportunity for corrective mannose supplementation

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Fig. 2

Mpi knockdown decreases full-length LLO and mannose-6-phosphate levels in zebrafish. (A) Full-length LLO (G₃M₉) levels are decreased by Mpi knockdown, as seen on FACE analysis. (B) G₃M₉ LLO (normalized to number of fish) and mannose-6-phosphate (M6P) levels (normalized to 100 microgram protein) in lysates from 4 dpf zebrafish larvae. Values from larvae injected with the standard control morpholino were set as 100%. Average from three experiments. (C) N-glycans are decreased to 53% in mpi morphants on FACE analysis as compared with standard controls (n=2 experiments).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-mpi
Knockdown Reagent:	MO1-mpi
Observed In:	dolichol-linked oligosaccharide biosynthetic process oligosaccharide-lipid intermediate biosynthetic process
Stage:	Day 4

Phenotype Detail

Acknowledgments

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