Fig. S6
fgf8a mutants exhibit no change in the number of rod photoreceptors or projection neurons. All images are dorsal views of confocal 3D reconstructions. WT larvae were co-labeled with foxd3:GFP and either Opsin-1 (rod photoreceptors outer segment) at 96 hpf or HuC/D (projection neurons) at 52 hpf. (A) Wild-type larvae exhibiting a parapineal (white arrowhead) and rod photoreceptors (blue arrowhead). (B) fgf8ax15 mutants have no parapineal organ but still form rod photoreceptors (blue arrowhead). (C) Wild-type embryos with visible parapineal (white arrowhead) and projection neurons (blue arrowheads). (D) fgf8ax15 mutants still develop projection neurons (blue arrowheads). Scale bar: 25 μm. |