Fig. S5
Fgf8a overexpression cannot rescue parapineal cell number defects in fgf8ax15 mutants. (A,B) Dorsal views of sox1a expression (black arrowheads) in fgf8ax15 mutants with and without the Tg[hsp70l:fgf8a]b1198 transgene at 36 hpf. (C,D) Dorsal views of gfi-1.2 expression (black arrowheads) in fgf8ax15 mutants with and without the Tg[hsp70l:fgf8a]b1198 transgene 52 hpf. Dashed line encircles pineal complex anlage in A-D. (E) Graph quantifying number of sox1a- and gfi-1.2-expressing cells in fgf8ax15 mutants with and without the Tg[hsp70l:fgf8a]b1198 transgene. (F) Graph quantifying the number of Tg[foxd3:GFP]- and Arr3a-positive cells in fgf8ax15 mutants with and without the Tg[hsp70l:fgf8a]b1198 transgene. Error bars represent s.e.m. n.s., not significant. Scale bar: 20 μm. |