FIGURE

Fig. 8

ID

ZDB-FIG-130109-35

Publication

Mosaliganti et al., 2012 - ACME: Automated Cell Morphology Extractor for Comprehensive Reconstruction of Cell Membranes

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Fig. 8

Robust correspondence between membrane and nuclear segmentations.

Algorithm performance was assessed by matching automated segmentations obtained from the nuclear and membrane channels. In the ideal case, each individual nucleus would match with a unique membrane and vice-versa. (A) A single 2D image plane is shown with contours of membrane and nuclear segmentations overlaid on raw data. Some cells have their corresponding nuclei located out-of-plane. The lack of a one-to-one correspondence indicates an error. For example, an over-segmentation of the membrane channel (white arrow) causes one of the membrane components to not contain a nucleus. (B) 3D renderings of cells from membrane and nuclear segmentations.

Expression Data

Genes:	Citrine Tomato
Fish:	hm25Tg hm26Tg
Anatomical Terms:	somite nucleus somite plasma membrane
Stage:	1-4 somites

Expression Detail

Gene	Antibody	Fish	Conditions	Stage	Qualifier	Anatomy	Assay
		hm26Tg	standard conditions	1-4 somites		somite plasma membrane	IFL
		hm25Tg	standard conditions	1-4 somites		somite nucleus	IFL

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Antibody Labeling

Phenotype Data

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Acknowledgments

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