Fig. 8 Robust correspondence between membrane and nuclear segmentations.
Algorithm performance was assessed by matching automated segmentations obtained from the nuclear and membrane channels. In the ideal case, each individual nucleus would match with a unique membrane and vice-versa. (A) A single 2D image plane is shown with contours of membrane and nuclear segmentations overlaid on raw data. Some cells have their corresponding nuclei located out-of-plane. The lack of a one-to-one correspondence indicates an error. For example, an over-segmentation of the membrane channel (white arrow) causes one of the membrane components to not contain a nucleus. (B) 3D renderings of cells from membrane and nuclear segmentations.