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Fig. S1

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ZDB-FIG-130108-22

Publication

Sharma et al., 2013 - PYK2: A calcium-sensitive protein tyrosine kinase activated in response to fertilization of the zebrafish oocyte

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Fig. S1

Effect of calcium on plasma membrane associated PTK activity. Zebrafish oocytes (1000) were fractionated and aliquots of the ‘light plasma membrane fraction’ (Wu and Kinsey, 2000) containing 10 μg protein were incubated in kinase assay buffer adjusted to contain different concentrations of CaCl₂ indicated at the top of the figure. Calmodulin (1 μM) was added to a fourth reaction (*). Samples were incubated at 37 °C for 1 min, after which SDS-sample buffer was added and samples were resolved by SDS-PAGE, treated with 0.5 M KOH,to preferentially hydrolyze P-Ser and P-Thr, then the remaining ³²PO₄—Tyr was detected by autoradiography. The position of Mr standards is indicated at left and the 120 KDa calcium/calmodulin-sensitive band is indicated by the arrow.

Expression Data

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Antibody Labeling

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Acknowledgments

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Reprinted from Developmental Biology, 373(1), Sharma, D., and Kinsey, W.H., PYK2: A calcium-sensitive protein tyrosine kinase activated in response to fertilization of the zebrafish oocyte, 130-140, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.