FIGURE

Fig. 2

ID

ZDB-FIG-130108-16

Publication

Sharma et al., 2013 - PYK2: A calcium-sensitive protein tyrosine kinase activated in response to fertilization of the zebrafish oocyte

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Fig. 2

Localization of activated PYK2 in the fertilized oocyte. Oocytes were fixed before and 2.5, 4.5, and 10 m.p.i., then processed for immunofluorescence, labeled with anti-PYK2-PY⁵⁷⁹ (upper panels) which would detect the activated form of PYK2 or with anti-PYK2 peptide (lower panels) which would detect the total pool of PYK2 protein. Bound antibody was detected with alexa 488-coupled secondary antibody (green) as described in “Materials and Methods”. Since the chorion formed a barrier to antibody diffusion, it was punctured multiple times with a micropipette after fixation or removed by dissection in samples fixed after it was fully elevated (10 m.p.i. and later). Magnification is indicated by the bar which represents 100 μm.

Expression Data

Antibodies:	Ab1-ptk2b Ab3-ptk2b
Fish:	WT
Anatomical Terms:	unfertilized egg whole organism
Stage:	1-cell

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 373(1), Sharma, D., and Kinsey, W.H., PYK2: A calcium-sensitive protein tyrosine kinase activated in response to fertilization of the zebrafish oocyte, 130-140, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.