Fig. 1

Detection of PYK2 in the oocyte plasma membrane; effect of fertilization. Plasma membrane fractions prepared at 7.5 min post-fertilization were analyzed by western blot and probed with an antibody to the mammalian PYK2 protein, anti-PY⁴⁰², anti-PY⁵⁷⁹, or with control rabbit IgG as described in ‘Materials and Methods’ (panel A). To detect changes in PYK2 phosphorylation after fertilization, plasma membrane samples prepared before (UF) and at 1, 2.5, and 10 min post-insemination (m.p.i.), were probed with the anti-PYK2 protein antibody and with the anti-PY⁵⁷⁹ antibody (panel B) to establish whether the amount or activation state of PYK2 associated with the plasma membrane changed in response to fertilization. Band intensity (‘Materials and Methods’) of the PYK2 PY⁵⁷⁹ labeled material in panel B was normalized to the PYK2 labeled bands in samples from five membrane preparations and is presented as a bar graph in panel C +/ S.E.M.