FIGURE

Fig. S1

ID

ZDB-FIG-121102-5

Publication

Hoffman et al., 2012 - An in vivo method to quantify lymphangiogenesis in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. S1

Zebrafish phenotype after vegfc morpholino injection or exposure to rapamycin or VEGFR-3 inhibitor. A–E, Representative bright field and green or red fluorescence microscopy images of whole Tg(fli1:EGFP)^y1 zebrafish after microangiography with Texas Red-LMD and Fluorescein-HMD*, including untreated 2-dpf and 3-dpf zebrafish, or 3-dpf zebrafish subjected to (C) vegfc MO (5 ng), (D) hVEGFR-3 inhibitor (30 mM) or (E) rapamycin (400 ng/ml). Note, all lymphangiogenesis inhibitors (C–E) led to pericardial edema (black arrows), but it was most prominent in zebrafish exposed to rapamycin. The latter also had significant yolk sac edema (black arrowhead), and an apparent collection of fluorescently-linked dextran in the posterior trunk (white arrows), suggesting a blood vessel leak versus lymphedema. *Fluorescein-HMD was co-injected with Texas Red-LMD during microangiography to enhance the green fluorescent signal emitted by the GFP-expressing zebrafish, as well as expose significant vascular leaks.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One