FIGURE

Fig. 2

ID

ZDB-FIG-121102-12

Publication

Hoffman et al., 2012 - An in vivo method to quantify lymphangiogenesis in zebrafish

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Fig. 2

Late-phase microangiography clearly demonstrates lymphatic capillaries in early zebrafish development.

A, Intracardiac injection technique employed for microangiography. The narrow red triangle represents the needle used to inject Texas Red-LMD into the zebrafish heart. The black box demarcates the approximate region of the trunk imaged. B and C, Microangiogram of 3-dpf Tg(fli1:EGFP)^y1 zebrafish. B, At 15 minutes post-injection (early-phase), Texas Red-LMD is primarily within ISVs (large white arrows), and lymphatic capillaries contain no Texas Red-LMD. C, By 4 hours post-injection (late-phase), most Texas Red-LMD has leaked from the blood vessels and collected at somitic boundaries (yellow arrowheads) or within deep lymphatic capillaries (small white arrows). Scale bar, 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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