Fig. 8

Gastrulation defects caused by p120 catenin δ1 knockdown are partially rescued by co-injection of high concentrations of constitutively active Rac1 GTPase mRNA (CA-Rac1 mRNA), but not with dominant-negative Rac1 mRNA (DN-Rac1 mRNA). A: Side views of embryos, dorsal up, anterior left, at the 11- to 12-somite stages uninjected and injected with Sp-MO and CA-Rac1 mRNA as indicated. B: Dorsal views of the same embryos as in A with anterior up. C: Percent embryos without gastrulation defects at Prim 5 and Prim 15 when injected with Sp-Mo and/or CA-Rac1 mRNA as indicated. D: Percent embryos without gastrulation defects at Prim 5 when injected with Sp-Mo and/or DN-Rac1 mRNA as indicated. E: Micrographs of dissected sibling embryos, dorsal views, anterior to the top right, analyzed with in situ hybridization using a myoD and papc (paraxial protocadherin C) combined probes at the 7-somite stages. Embryos were uninjected or injected as indicated. F: Summary of the average differences in width of the second-to-last somite of embryos treated and probed as in E. Somite numbers are indicated below the bars. G: Summary of the average difference in width of the paraxial mesoderm at the conditions in E and measurements as indicated in Figure 4. The numbers of embryos are given on the graph bars. Scale bars (A, B) = 200 μm and (C) = 100 μm.