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ZFIN ID: ZDB-FIG-121023-19
Yang et al., 2012 - Neutrophils exert protection in the early tuberculous granuloma by oxidative killing of mycobacteria phagocytosed from infected macrophages. Cell Host & Microbe   12(3):301-312 Full text @ Cell Host Microbe
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 1

Zebrafish Neutrophils Are Marked by GFP Expression in the lyz-Green Line and Are Functionally Similar to Mammalian Neutrophils (A) Cartoon of the zebrafish at two developmental stages showing sites of primitive and definitive hematopoiesis (black arrows) and injection sites used in this study (red arrows). CHT, caudal hematopoietic tissue. (B) GFP-positive cells in lyz-green fish showing well-defined granules (black brackets). Scale bar, 10 μm. (C) Colocalization of GFP-expressing cells (black arrows) with Sudan black. Scale bar, 20 μm. (D) Individual fluorescence channels of a single image of the lyz-red mpx:gfp double transgenic larvae. Arrow identifies a cell with differential expression of the two transgenes. Scale bar, 20 μm. (E) DIC (top) and GFP fluorescence channel (bottom) of a single image of a lyz-green larva stained with neutral red. Macrophages, red arrows; neutrophil, green arrowhead; pigment cell, P. Scale bar, 20 μm. (F) Whole-mount in situ hybridization analysis of 48 hpf lyz-green larva with fms probe (red fluorescence) combined with GFP antibody staining (green fluorescence). Macrophages, yellow arrowheads; neutrophils, yellow arrows. Scale bar, 10 μm. (G–J) Median phagocyte recruitment into the ear (G) or hindbrain ventricle (H–J) of 48 hpf larvae in response to injection of vehicle (mock), IL-8, LTB4, or fMLP. In (I) and (J), fpr1 mo refers to larvae with morpholino knockdown of the formyl peptide receptor 1. Results in (G)–(J) are representative of two experiments. Significance testing performed by Kruskal-Wallis test with Dunn′s posttest in (G)–(J). *p < 0.05; **p < 0.01; ***p < 0.0001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
csf1ra nz117Tg standard conditions Long-pec macrophage ISH
DsRed2 i114Tg; nz50Tg standard conditions Long-pec neutrophil IFL
EGFP nz117Tg standard conditions Long-pec Not Detected macrophage IFL
Long-pec Not Detected macrophage IHC
Long-pec neutrophil IHC
Long-pec neutrophil IFL
GFP i114Tg; nz50Tg standard conditions Long-pec neutrophil IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-fpr1 standard conditions Long-pec macrophage activation involved in immune response occurrence, normal
Long-pec neutrophil activation involved in immune response disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Host & Microbe for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Host Microbe