FIGURE

Fig. 5

ID

ZDB-FIG-120907-11

Publication

Xing et al., 2012 - Zebrafish foxP2 Zinc Finger Nuclease Mutant Has Normal Axon Pathfinding

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

foxP2 does not affect axon pathfinding.

Confocal z-stack images of whole-mount embryos, scale bars 50 μm, show no difference between wild-type and foxP2 mutant (/) embryos for axon pathfinding using a variety of axonal labels. (A–D) anti-SV2 immunohistochemistry at 24hpf, lateral views of the brain, rostral to the left (A, B) and 72hpf, dorsal views of the spinal cord, rostral to the top (C, D). (E, F) anti-acetylated tubulin immunohistochemistry at 72hpf, ventral views of the optic chiasm. (G-L) GFP immunohistochemistry at 72hpf in Tg(foxP2-enhancerA.2:egfp-caax) embryos that labels foxP2 neurons show no pathfinding errors in anterior commissure (ac), longitudinal commissures (lc), tract of the commissure of the posterior tuberculum (TCPTc), or reticulospinal axons (rs). (M-R) GFP immunohistochemistry at 72hpf in Tg(otpb.A:egfp-caax) embryos for visualization of dopaminergic and neuroendocrine projections (M, N, with insets shown in O, P) in the brain, and dopaminergic axon tracts in spinal cord (Q, R).

Expression Data

Gene:	EGFP
Antibody:	Ab-SV2
Fish:	WT zc49Tg zc69Tg foxp2^zc82/+; foxp2^zc84/+ foxp2^zc82/+; foxp2^zc84/+; zc49Tg foxp2^zc82/+; foxp2^zc84/+; zc69Tg
Anatomical Terms:	anterior commissure brain axon commissure of the caudal tuberculum dopaminergic neuron axon neuroendocrine cell axon reticular formation axon spinal cord axon
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One