header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-120907-11
Xing et al., 2012 - Zebrafish foxP2 Zinc Finger Nuclease Mutant Has Normal Axon Pathfinding. PLoS One   7(8):e43968 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Protruding-mouth

Fig. 5

foxP2 does not affect axon pathfinding.

Confocal z-stack images of whole-mount embryos, scale bars 50 μm, show no difference between wild-type and foxP2 mutant (/) embryos for axon pathfinding using a variety of axonal labels. (A–D) anti-SV2 immunohistochemistry at 24hpf, lateral views of the brain, rostral to the left (A, B) and 72hpf, dorsal views of the spinal cord, rostral to the top (C, D). (E, F) anti-acetylated tubulin immunohistochemistry at 72hpf, ventral views of the optic chiasm. (G-L) GFP immunohistochemistry at 72hpf in Tg(foxP2-enhancerA.2:egfp-caax) embryos that labels foxP2 neurons show no pathfinding errors in anterior commissure (ac), longitudinal commissures (lc), tract of the commissure of the posterior tuberculum (TCPTc), or reticulospinal axons (rs). (M-R) GFP immunohistochemistry at 72hpf in Tg(otpb.A:egfp-caax) embryos for visualization of dopaminergic and neuroendocrine projections (M, N, with insets shown in O, P) in the brain, and dopaminergic axon tracts in spinal cord (Q, R).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP foxp2zc82/+; foxp2zc84/+; zc49Tg standard conditions Protruding-mouth dopaminergic neuron axon IHC
Protruding-mouth neuroendocrine cell axon IHC
Protruding-mouth spinal cord axon IHC
foxp2zc82/+; foxp2zc84/+; zc69Tg standard conditions Protruding-mouth anterior commissure IHC
Protruding-mouth commissure of the caudal tuberculum IHC
Protruding-mouth reticular formation axon IHC
zc49Tg standard conditions Protruding-mouth dopaminergic neuron axon IHC
Protruding-mouth neuroendocrine cell axon IHC
Protruding-mouth spinal cord axon IHC
zc69Tg standard conditions Protruding-mouth anterior commissure IHC
Protruding-mouth commissure of the caudal tuberculum IHC
Protruding-mouth reticular formation axon IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-SV2 IHC WT standard conditions Prim-5 brain axon
IHC Protruding-mouth spinal cord axon
IHC foxp2zc82/+; foxp2zc84/+ standard conditions Prim-5 brain axon
IHC Protruding-mouth spinal cord axon
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One