FIGURE

Fig. 3

ID

ZDB-FIG-120824-30

Publication

Won et al., 2012 - Characterization of na(+) and ca(2+) channels in zebrafish dorsal root ganglion neurons

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Fig. 3

Immunohistochemistry of DRG neurons in juvenile isl2b:EGFP fish sections.

A, Confocal image displaying EGFP-labeled DRG neurons and DFL in transverse section. Note that both dorsal and ventrolateral positioned DRG (arrows) cell bodies were observed. B, Higher magnification images of DRG ganglion reveal a variety of cell body sizes (B-a). Counterstaining with DAPI (B-b, magenta) revealed that some EGFP-negative or dim cell bodies were also present in DRG sections (asterisks). C, EGFP-positive and -negative DRG neurons from juvenile isl2b:EGFP fish. A few DRG neurons stained with anti-HuC/HuD neuronal protein antibody (magenta) did not express EGFP. D, Isolectin B₄ (IB₄) staining. Most DRG cell bodies and some spinal neurons were labeled with IB₄ (magenta). Dashed rectangular region was enlarged for displaying labeled DRG neurons (D-a). Solid line represents the dorsal boundary of the spinal cord. Inset cartoons represent orientation of images. D, dorsal; V, ventral; SC, spinal cord; DFL, dorsal longitudinal fasciculus. All scale bars represent 10 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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