FIGURE

Fig. 7

ID

ZDB-FIG-120814-42

Publication

Abrams et al., 2012 - Dynamic Assembly of Brambleberry Mediates Nuclear Envelope Fusion during Early Development

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Fig. 7

Bmb Is Required for Pronuclear Fusion(A) At 16 mpf, WT pronuclei are fully congressed.(B) Some WT pronuclei begin to fuse at 16 mpf.(C) At 19 mpf, WT pronuclear fusion is complete.(D) At 22 mpf, in WT embryos a single condensed DNA mass is detected at the first prometaphase.(E) At 16 mpf, bmb pronuclei are fully congressed.(F) At 19 mpf, bmb pronuclei fail to fuse as chromatin begins to condense.(G) At 22 min, two separate condensed DNA masses are detected in bmb embryos at the first prometaphase. Scale bars, 10 μm. At each time point, n e 4. Images correspond to Z-projections of individual confocal Z-planes. WT TL males were crossed to female bmb mutants in (E)–(G).

Expression Data

Gene:	bmb
Antibody:	Ab1-bmb
Fish:	WT bmb^{p22atuz/p22atuz} (TL)
Anatomical Term:	cell pronucleus
Stage:	1-cell

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	bmb^{p22atuz/p22atuz} (TL)
Observed In:	cell pronucleus pronuclear fusion
Stage:	1-cell

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 150(3), Abrams, E.W., Zhang, H., Marlow, F.L., Kapp, L., Lu, S., and Mullins, M.C., Dynamic Assembly of Brambleberry Mediates Nuclear Envelope Fusion during Early Development, 521-532, Copyright (2012) with permission from Elsevier. Full text @ Cell