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ZIRC
ZFIN ID: ZDB-FIG-120725-30
Powell et al., 2012 - Genomic organisation, embryonic expression and biochemical interactions of the zebrafish junctional adhesion molecule family of receptors. PLoS One   7(7):e40810 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Prim-5

Fig. 1

Cloning of putative jama2 and jamc2 paralogues by 3′ RACE or RT-PCR.

Agarose gel electrophoresis results of amplification of full-length jama2 by 3′ RACE (A) or jamc2 by RT-PCR (B) from cDNA prepared from total RNA extracts of wild-type 24 h. p. f. zebrafish embryos. (A) Lanes: - 3′ RACE negative control; 1–4 igsf11 positive control: 3′ RACE –1 5′ primer, 2 nested 5′ primer; nested PCR –3 negative control, 4 nested 5′ primer; 5–8 jama2:3′ RACE –5 5′ primer, 6 nested 5′ primer; nested PCR –7 negative control, 8 nested 5′ primer. (B) Lanes: - negative control; + ef1α positive control; 1 jamb positive control; 2 & 4 jamc2 primers designed to amplify predicted full-length ORF; 3 & 5 jamc2 primers designed to amplify IgSF domains. M, DNA size markers with size of selected bands indicated in kbp (left).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
f11r.2 WT standard conditions Prim-5 whole organism RTPCR
jam3a WT standard conditions Prim-5 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One