ZFIN ID: ZDB-FIG-120724-17
Flowers et al., 2012 - A zebrafish Notum homolog specifically blocks the Wnt/beta-catenin signaling pathway. Development (Cambridge, England)   139(13):2416-2425 Full text @ Development
Anatomical Terms:
Stage Range: 50%-epiboly to Prim-5

Fig. 1 Expression of zebrafish notum1a. (A-E) Expression of zebrafish notum1a. (A) At 50% epiboly, notum1a is expressed around the entire blastoderm margin. (B) A lateral view of notum1a expression at tail bud reveals expression in the lateral neural plate (asterisk). (C) A flat-mount of the tail bud displays notum1a expression in the posterior neural plate, particularly at the lateral edges (asterisk) and midline. (D) At six somites, as neurulation proceeds, lateral neural plate notum1a expression becomes more medially located. More anteriorly, notum1a begins to appear in the midbrain-hindbrain boundary (mhb, arrow) and rhombomeres 3 and 5 (r3, r5, arrowheads). (E) At 24 hpf, notum1a is expressed in the mhb (arrow) and in the dorsal neural tube, as revealed in a section through the trunk at the level of the yolk extension (line, inset). Scale bar: 50 μm. (F,G) Wnt/β-catenin signaling is needed for notum1a expression. (F) Mosaically expressed activated β-catenin (red) colocalizes with ectopic notum1a expression (arrowheads) at 80% epiboly. Scale bar: 50 μm. (G,H) Although normally expressed in heat-shocked non-transgenic embryos at 22 hpf (G), notum1a is absent in heat-shocked Tg(hsp70:tcf3-gfp)w26/+ embryos (H) except in small domain within the tail bud 2 hours post-heat shock. Scale bars in A-D,G,H: 200 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
notum1a AB/TU standard conditions 50%-epiboly blastoderm anatomical margin ISH
Bud axis ISH
Bud neural plate lateral region ISH
Bud posterior neural plate ISH
5-9 somites neural plate lateral region ISH
5-9 somites presumptive rhombomere 3 ISH
5-9 somites presumptive rhombomere 5 ISH
Prim-5 central nervous system ISH
Prim-5 midbrain hindbrain boundary ISH
Prim-5 neural tube ISH
AB/TU heat shock 26+ somites central nervous system ISH
26+ somites midbrain hindbrain boundary ISH
26+ somites neural tube ISH
26+ somites tail bud ISH
w26Tg heat shock 26+ somites Not Detected central nervous system ISH
26+ somites Not Detected midbrain hindbrain boundary ISH
26+ somites Not Detected neural tube ISH
26+ somites tail bud ISH
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development