ZFIN ID: ZDB-FIG-120724-12
Flowers et al., 2012 - A zebrafish Notum homolog specifically blocks the Wnt/beta-catenin signaling pathway. Development (Cambridge, England)   139(13):2416-2425 Full text @ Development
Knockdown Reagent:
Anatomical Term:
Stage: Prim-5

Fig. S6 Loss of Notum1a results in expanded Wnt reporter activity, but not bmp4, foxa, pax3a or pax6a expression. (A,B) Relative to that in uninjected controls (A), the domain of egfp expression in the tails of embryos in the Tg(TOP:dEGFP)w26 reporter line is expanded in notum1a MO-injected embryos (B) at 24 hpf. (C-L) Transverse sections of neural tubes at 24 hpf. bmp4 (C,D), pax3a (E,F), pax6a (G,H), lhx3 (I,J) and foxa (K,L) are not affected in notum1a MO-injected embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP w26Tg control Prim-5 tail bud ISH
w26Tg + MO2-notum1a standard conditions Prim-5 tail bud ISH
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development