Fig. 2

Morpholino-mediated gene knockdown of sema3d and its putative receptors. In all graphs, MO represents the particular gene-targeting morpholino; MM represents the particular 5 mis-match/control morpholino; UN represents uninjected/untreated fins. (A) Total regenerate length was measured. sema3d-knockdown and plxna3-knockdown cause reduced fin length (*); nrp2a-knockdown causes increased fin length (*), (B) the total number of H3P positive cells were counted. sema3d-knockdown causes reduced levels of cell proliferation (N); nrp2a-knockdown causes increased levels of cell proliferation (*), (C) segment length was measured in treated wild-type fins. sema3d-knockdown and plxna3-knockdown cause reduced segment length (*) and (D) segment length was measured in treated alf^dty86 fins. sema3d-knockdown and plxna3-knockdown cause reduced segment length and rescue joint formation in alf^dty86 (*). Statistically different data sets (*) were determined by the student′s t-test where p<0.05. By the student′s t-test, there is no statistical difference between MM and UN for any mismatch morpholino. Error bars represent the standard deviation.