ZFIN ID: ZDB-FIG-120710-11
Soares et al., 2012 - Dre-miR-2188 Targets Nrp2a and Mediates Proper Intersegmental Vessel Development in Zebrafish Embryos. PLoS One   7(6):e39417 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec

Fig. 3 ISH of Nrp2a after miR-2188 deregulation.

A) After injection of miR-2188 duplex, Nrp2a detection decreased between the somite boundaries at 24 hpf. On the other hand, in miR-2188 morphants, Nrp2a detection was more prounounced throughout the trunk and tail region, between the somite boundaries, when compared with the control. B) At 48 hpf and after miR-2188 overexpression, Nrp2a was not detected in the blood vessels or in the PCV when compared with the control embryos. On the other hand, miR-2188 morphants express Nrp2a in the trunck and tail, especially between the somite boundaries, which is not detected in 48 hpf control embryos. All embryos are in lateral view, anterior to left. Arrows indicate hybridization signal. Microscopic pictures were taken with a 6,3x magnification.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
nrp2a AB control Long-pec Not Detected post-vent region ISH
Long-pec Not Detected somite border ISH
Long-pec Not Detected trunk ISH
AB + MO1-mir2188 standard conditions Prim-5 post-vent region ISH
Prim-5 somite border ISH
Prim-5 trunk ISH
Long-pec post-vent region ISH
Long-pec somite border ISH
Long-pec trunk ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One