FIGURE

Fig. S1

ID

ZDB-FIG-120508-16

Publication

Stewart et al., 2012 - Limited dedifferentiation provides replacement tissue during zebrafish fin regeneration

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Fig. S1

Antibody validation. (A–D) An anti-dsRed antibody detects mCherry expression in sectioned fins. A section from a Class 1 mosaic Tg(dusp6:Cre-ERT2, EAB:EGFP-FlExmCherry) fin immunostained with an anti-dsRed antibody (A, green) with mCherry expression shown in red (B). Nuclei are stained with Hoechst (C, blue) and the overlay is shown (D). All mCherry⁺ cells are positive for dsRed (white arrows). (E–L) The zns-5 and zn-3 antibodies stain osteoblasts. Sections of non-regenerating caudal fins from Tg(sp7:EGFP) fish showing EGFP expression (E, I green) and stained with zns-5 or zn-3 antibodies (F, J, red) and Hoechst (G, K, blue). The overlays (H, L) demonstrate co-localization of EGFP⁺ cells with zns-5⁺ or zn-3⁺ cells, respectively (cells marked with white arrows).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 365(2), Stewart, S., and Stankunas, K., Limited dedifferentiation provides replacement tissue during zebrafish fin regeneration, 339-349, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.