FIGURE

Fig. 3

ID

ZDB-FIG-120508-10

Publication

Stewart et al., 2012 - Limited dedifferentiation provides replacement tissue during zebrafish fin regeneration

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Fig. 3

Epidermal cells do not contribute to the blastema or change fate during regeneration. (A–L) Longitudinal sections of the caudal fin of Tg(dusp6:Cre-ERT2, EAB:EGFP-FlEx-mCherry) Class 1 mosaic animals demonstrating mCherry expression (red, A, E, I) and stained with anti-p63 antibodies to mark epidermal cells (green, B, F, J) and with Hoechst to mark nuclei (blue, C, G, K). The three-color overlays are shown in (D, H, L). (A–D) mCherry⁺ epidermal cells in Tg(dusp6:Cre-ERT2, EAB:EGFP-FlEx-mCherry) Class 1 mosaic animals remain positive for the epidermal maker p63 at 2 dpa. The yellow dashed line marks the approximate amputation site and the white dashed line marks the boundary between the epidermis and the blastema. (E–L) Stained fin sections from the same Tg(dusp6:Cre-ERT2, EAB:EGFP-FlEx-mCherry) Class 1 mosaic animal prior to amputation (E–H) and 7 dpa (I–L). White arrows indicate mCherry⁺/p63⁺ epidermis.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 365(2), Stewart, S., and Stankunas, K., Limited dedifferentiation provides replacement tissue during zebrafish fin regeneration, 339-349, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.