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General Information
ZIRC
ZFIN ID: ZDB-FIG-120426-4
Simms et al., 2012 - Modelling a ciliopathy: Ahi1 knockdown in model systems reveals an essential role in brain, retinal, and renal development. Cellular and molecular life sciences : CMLS   69(6):993-1009 Full text @ Cell. Mol. Life Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: 5-9 somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: 5-9 somites

Fig. 4 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP zf106Tg control 5-9 somites Kupffer's vesicle cell-cell junction IFL
zf106Tg + MO1-ahi1 standard conditions 5-9 somites Kupffer's vesicle cell-cell junction IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-prkcz IHC slc24a5b1/b1 control 5-9 somites Kupffer's vesicle apical plasma membrane
IHC slc24a5b1/b1 + MO1-ahi1 standard conditions 5-9 somites Kupffer's vesicle apical plasma membrane
Ab1-tuba IHC slc24a5b1/b1 control 5-9 somites Kupffer's vesicle cilium
IHC slc24a5b1/b1 + MO1-ahi1 standard conditions 5-9 somites Not Detected Kupffer's vesicle
IHC zf106Tg control 5-9 somites Kupffer's vesicle cilium
IHC zf106Tg + MO1-ahi1 standard conditions 5-9 somites Not Detected Kupffer's vesicle
Phenotype Details
Fish Conditions Stage Phenotype
slc24a5b1/b1 + MO1-ahi1 standard conditions 5-9 somites Kupffer's vesicle lacks all parts of type ciliated epithelial cell cilium, abnormal
5-9 somites motile cilium assembly decreased process quality, abnormal
zf106Tg + MO1-ahi1 standard conditions 5-9 somites Kupffer's vesicle lacks all parts of type ciliated epithelial cell cilium, abnormal
Full text @ Cell. Mol. Life Sci.