FIGURE

Fig. 6

ID

ZDB-FIG-120417-11

Publication

Voz et al., 2012 - Fast Homozygosity Mapping and Identification of a Zebrafish ENU-Induced Mutation by Whole-Genome Sequencing

Other Figures

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Fig. 6

A: Time course analysis of zebrafish snapc4 expression by semi-quantitative RT-PCR.

A control without addition of RNA (lane -) was included as a negative control. 3 μg of total RNA extracted at each time point were loaded on a denaturating agarose gel to check the quality and the quantity of the RNA used for each RT-PCR. B: Expression profile of the snapc4 transcripts performed by WISH at 15 hpf (12S), 24 hpf, 3 dpf and 4 dpf. C: Translation-blocking snapc4 morpholino leads to growth retardation before 24 hpf. Bright-field images of 24 hpf embryos injected with 8 ng of control morpholino or of MO_ATG-snapc4 : 75% of the embryos injected with MO_ATG-snapc4 show severe growth retardation compared to the control morphant.

Expression Data

Gene:	snapc4
Fish:	AB
Anatomical Terms:	brain eye gut mandibular arch skeleton whole organism
Stage Range:	4-cell to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO6-snapc4
Knockdown Reagent:	MO6-snapc4
Observed In:	chordate embryonic development whole organism
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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