ZFIN ID: ZDB-FIG-120329-48
Royo et al., 2012 - Identification and Analysis of Conserved cis-Regulatory Regions of the MEIS1 Gene. PLoS One   7(3):e33617 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 4

Pax6 regulates the enhancer activity of HHc2:066650.

A) Representative pictures of transgenic zebrafish embryos at 24 and 48 hpf, corresponding to HHc2:066650 F2 offspring injected with MOpaxA, MOpaxB, or a mixture of both. The red arrow points to the retina, where a strong decrease in the GFP levels is observed after morpholino treatment. A control mopholino-injected individual is shown for comparison. B) Representative mosaic embryos injected with HHc2:066650 construct with/without the two candidate Pax6 binding sites. Both the GFP and RFP channels are included. The boxed region is magnified on the right. The dashed circle delineates the eye. (C) GFP expression was measured in the eye (area marked by the dashed circle in (B)) of wild type and Δpax6_BS version of HHc2:066650 mosaic embryos (n = 9 and 11 embryos, respectively), and normalized with their respective muscle RFP expression. Enhancer signal significantly decreased in the mutant version of HHc2:066650 (p = 0.018, Mann Whitney test). The box plots contains a central rectangle, which spans from the first quartile to the third quartile. A segment inside the rectangle shows the median and whiskers above and below the box show the locations of the minimum and maximum.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP upo16Tg standard conditions Prim-5 eye RTPCR
Long-pec eye RTPCR
upo16Tg + MO2-pax6a standard conditions Prim-5 eye RTPCR
Long-pec eye RTPCR
upo16Tg + MO2-pax6a + MO2-pax6b standard conditions Prim-5 eye RTPCR
Long-pec eye RTPCR
upo16Tg + MO2-pax6b standard conditions Prim-5 eye RTPCR
Long-pec eye RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
upo16Tg + MO2-pax6a standard conditions Prim-5 eye EGFP expression decreased amount, abnormal
Long-pec eye EGFP expression decreased amount, abnormal
upo16Tg + MO2-pax6a + MO2-pax6b standard conditions Prim-5 eye EGFP expression decreased amount, abnormal
Long-pec eye EGFP expression decreased amount, abnormal
upo16Tg + MO2-pax6b standard conditions Prim-5 eye EGFP expression decreased amount, abnormal
Long-pec eye EGFP expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One