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ZFIN ID: ZDB-FIG-120316-108
Kirchmaier et al., 2012 - The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development. Developmental Biology   363(2):438-450 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 7 Cardiac dysmorphogenesis in the popdc2 morphant. Zebrafish embryos were injected with 2 ng/embryo of (A,C,E) control morpholino (MO-CTR) or (B,D,F) MO1/MO2 popdc2 morpholinos. (A,B) Confocal analysis of 3 dpf transgenic Tg(cmlc2:GFP) hearts. The heart of the popdc2 morphants is dysmorphic and lacks trabeculation. (C,D) Anti-Alcam antibody staining reveals normal cell shape in the popdc2 morphants. (E,F) Anti-tropomyosin antibody staining shows a reduction in myofibrillar content in cardiac myocytes of the popdc2 morphants. Abbreviations: A, atrium; V, ventricle. Scale bar = 10 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP twu34Tg control Protruding-mouth atrium IFL
Protruding-mouth cardiac ventricle IFL
twu34Tg + MO1-popdc2 + MO2-popdc2 standard conditions Protruding-mouth atrium IFL
Protruding-mouth cardiac ventricle IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab4-tpm IHC WT control Protruding-mouth cardiac muscle cell
IHC WT + MO1-popdc2 + MO2-popdc2 standard conditions Protruding-mouth cardiac muscle cell
zn-8 IHC WT control Protruding-mouth cardiac muscle cell
IHC WT + MO1-popdc2 + MO2-popdc2 standard conditions Protruding-mouth cardiac muscle cell
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-popdc2 + MO2-popdc2 standard conditions Protruding-mouth atrium orientation cardiac ventricle, abnormal
Protruding-mouth cardiac muscle cell shape, normal
Protruding-mouth cardiac muscle cell myofibril decreased amount, abnormal
Protruding-mouth heart malformed, abnormal
Protruding-mouth heart looping process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 363(2), Kirchmaier, B.C., Poon, K.L., Schwerte, T., Huisken, J., Winkler, C., Jungblut, B., Stainier, D.Y., and Brand, T., The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development, 438-450, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.