FIGURE

Fig. 4

ID

ZDB-FIG-120315-48

Publication

Simmons et al., 2012 - Mutation of pescadillo Disrupts Oligodendrocyte Formation in Zebrafish

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Fig. 4

Markers of cell cycle activity are significantly increased in the spinal cords of pes^vu166-/- embryos.

(A–H) Transverse sections through the spinal cords of 3 dpf (A,B), 4 dpf (C,D) and 2.5 dpf (E–H) larvae processed for immunohistochemistry. (A,C) Wild type spinal cord (dashed line) section showing BrdU⁺ (arrows) and BrdU^- (arrowheads) olig2⁺, Sox10⁺ OPCs. (B,D) pes^{vu166 -/-} spinal cord sections. OPCs did not incorporate BrdU but numerous cells lining the central canal and medial septum were BrdU⁺. (E,F) Representative spinal cord sections showing the M-phase marker PH-3. (G,H) Representative spinal cord sections showing TUNEL labeling (red) to mark apoptotic cells. DAPI labeling (blue) marks cell nuclei. (I) Quantification of the change in total BrdU⁺ and PH-3⁺ cells between wild-type and pes^{vu166 -/-} spinal cords. (J) Quantification of DAPI⁺ and TUNEL⁺ cells in wild-type and mutant spinal cords. Asterisk (*) indicates pj0.05 by Student′s T-test.

Expression Data

Genes:	EGFP sox10
Antibody:	Ab1-sox10
Fish:	vu12Tg pes^vu166/vu166; vu12Tg
Anatomical Term:	oligodendrocyte
Stage Range:	Pec-fin to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pes^vu166/vu166; vu12Tg
Observed In:	apoptotic process cell cycle process cell population proliferation spinal cord
Stage:	Pec-fin

Phenotype Detail

Acknowledgments

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