Fig. 1
- ID
- ZDB-FIG-120315-45
- Publication
- Simmons et al., 2012 - Mutation of pescadillo Disrupts Oligodendrocyte Formation in Zebrafish
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Characterization and genetic mapping of the vu166 mutant allele. Lateral views of 4 dpf wild-type (A,C) and vu166 -/- larvae (B,D). EGFP expression revealed oligodendrocytes in dorsal spinal cord of wild-type Tg(olig2:EGFP) larva (C, bracket). Fewer dorsal EGFP+ cells were evident in a vu166 -/- larva (D). Bulked segregant analysis revealed that the vu166 allele links to chromosome 5 (E). The recombination frequencies (i) at various microsatellite markers (ii) at known locations (iii) along chromosome 5 show that the mutation lies near the pescadillo (pes) gene locus. The vu166 allele failed to complement the previously characterized peshi2 mutant allele, producing phenotypically wild-type (F) and mutant (G) larvae in a 3:1 ratio. The Pes gene product (H) contains a highly conserved Pescadillo Homology domain, a BRCA1 C-terminal domain, two Acidic Domains (AD), and a Nuclear Localization Sequence (NLS). Sequencing of pes cDNA obtained from vu166 mutants revealed a single base pair mutation that creates a premature stop codon predicted to prematurely truncate the protein. |
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Stage: | Day 4 |
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Stage: | Day 4 |