ZFIN ID: ZDB-FIG-120315-33
Clendenon et al., 2012 - Zebrafish cadherin-11 participates in retinal differentiation and retinotectal axon projection during visual system development. Developmental dynamics : an official publication of the American Association of Anatomists   241(3):442-454 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Day 4

Fig. 5

cdh11 knockdown inhibits retinal differentiation. A–C: Zebrafish embryos were injected with standard control morpholino oligonucleotide (MO, Control columns) or cdh11 MO (Slight, Moderate, and Severe columns). A: In 2 day postfertilization (dpf) embryos, retinal ganglion cells were visualized using whole-mount zn-5 monoclonal antibody staining and confocal imaging. Ventral view projections of sections through the optic nerve show retinal ganglion cells and their axon processes. In cdh11 morphant embryos, the retinal ganglion cell layer was progressively reduced in thickness and numbers of ganglion cells detected. B: In 3 dpf embryos, cryostat sections were immunolabeled using antibodies that detect retinal layers: HuC/D (HU), which detects retinal ganglion cells and inner nuclear layer neurons; zn-5 (Zn5), which detects retinal ganglion cells and their axons; Pax6 (Pax6), which detects cells in the ganglion cell layer and brightly labeled amacrine cells within the inner nuclear layer (arrowhead in Control image); and zpr-1 (Zpr1), which detects double cone photoreceptors. Cdh11 loss-of-function results in a progressive reduction in immunostaining markers for differentiated cell types in the retina. C: In 4 dpf embryos, cryostat sections were immunolabeled using antibodies that detect retinal layers: Pax6 (Pax6), which detects a variety of cells including brightly labeled amacrine cells within the inner nuclear layer (arrowhead in Control image); and zpr-1 (Zpr1), which detects double cone photoreceptors. The older embryos maintain the Cdh11 loss-of-function phenotype, showing reduced labeling of differentiated cell types. Scale bar = 100 μp>

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-cdh11 standard conditions Long-pec cranial nerve II physical object quality, abnormal
Long-pec retina development in camera-type eye disrupted, abnormal
Long-pec retinal ganglion cell layer decreased thickness, abnormal
Long-pec retinal ganglion cell layer disorganized, abnormal
Long-pec retinal ganglion cell layer has fewer parts of type retinal ganglion cell, abnormal
Protruding-mouth photoreceptor cell differentiation decreased process quality, abnormal
Protruding-mouth retina development in camera-type eye disrupted, abnormal
Protruding-mouth retinal ganglion cell layer has fewer parts of type retinal ganglion cell, abnormal
Protruding-mouth retinal inner nuclear layer has fewer parts of type amacrine cell, abnormal
Day 4 photoreceptor cell differentiation decreased process quality, abnormal
Day 4 retina development in camera-type eye disrupted, abnormal
Day 4 retinal inner nuclear layer has fewer parts of type amacrine cell, abnormal
WT + MO2-cdh11 standard conditions Long-pec cranial nerve II physical object quality, abnormal
Long-pec retina development in camera-type eye disrupted, abnormal
Long-pec retinal ganglion cell layer decreased thickness, abnormal
Long-pec retinal ganglion cell layer disorganized, abnormal
Long-pec retinal ganglion cell layer has fewer parts of type retinal ganglion cell, abnormal
Protruding-mouth photoreceptor cell differentiation decreased process quality, abnormal
Protruding-mouth retina development in camera-type eye disrupted, abnormal
Protruding-mouth retinal ganglion cell layer has fewer parts of type retinal ganglion cell, abnormal
Protruding-mouth retinal inner nuclear layer has fewer parts of type amacrine cell, abnormal
Day 4 photoreceptor cell differentiation decreased process quality, abnormal
Day 4 retina development in camera-type eye disrupted, abnormal
Day 4 retinal inner nuclear layer has fewer parts of type amacrine cell, abnormal
WT + MO3-cdh11 standard conditions Long-pec cranial nerve II physical object quality, abnormal
Long-pec retina development in camera-type eye disrupted, abnormal
Long-pec retinal ganglion cell layer decreased thickness, abnormal
Long-pec retinal ganglion cell layer disorganized, abnormal
Long-pec retinal ganglion cell layer has fewer parts of type retinal ganglion cell, abnormal
Protruding-mouth photoreceptor cell differentiation decreased process quality, abnormal
Protruding-mouth retina development in camera-type eye disrupted, abnormal
Protruding-mouth retinal ganglion cell layer has fewer parts of type retinal ganglion cell, abnormal
Protruding-mouth retinal inner nuclear layer has fewer parts of type amacrine cell, abnormal
Day 4 photoreceptor cell differentiation decreased process quality, abnormal
Day 4 retina development in camera-type eye disrupted, abnormal
Day 4 retinal inner nuclear layer has fewer parts of type amacrine cell, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.