FIGURE

Fig. 9

ID

ZDB-FIG-120228-15

Publication

Otten et al., 2012 - Xirp proteins mark injured skeletal muscle in zebrafish

Other Figures

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Fig. 9

Efficient Morpholino antisense oligonucleotide-mediated knock-down of xirp2a does not affect myofibrillogenesis.

(A) Phenotypically, xirp2a morphants are indistinguishable from WT embryos at 24 hpf. Scale bar: 250 μm. (B) Efficient gene knock-down of xirp2a is assessed by immunohistochemistry at 24 hpf. Green: Xirp2a; red: Actin. Scale bar: 20 μm. (C) Complete loss of Xirp2a does not affect myofibrillogenesis and correct sarcomeric organization of skeletal muscle as determined by immunohistochemistry using an antibody against the A/I junction epitope of Titin and rhodamine phalloidin to label sarcomeric Actin at 24 hpf. Green: Titin; red: Actin. Scale bar: 5 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-xirp2a
Knockdown Reagent:	MO1-xirp2a
Observed In:	myofibril assembly sarcomere organization
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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