ZFIN ID: ZDB-FIG-120227-4
Odenthal et al., 1996 - Mutations affecting the formation of the notochord in the zebrafish, Danio rerio. Development (Cambridge, England)   123:103-115
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Bud to 10-13 somites

Fig. 3

Dorsal views of (A-E) tailbud stage and (F-J) 10-somite stage embryos. (A-C, F-H) Whole-mount Ntl antibody staining; (D,E) twi in situ hybridization (blue) and Ntl antibody staining (brown); (I, J) shh in situ hybridization (blue) and Ntl antibody staining (brown). Anterior is to the left. (A,D,F,I) Wild type, (B) flhtk241, (C) momth211, (E) ntltc41, (G) heterozygous and (H, J) homozygous doctt258 embryos. Ntl expression in the axial mesoderm is reduced in (B) flh and (C) mom embryos at the tailbud stage. Expression of twi in (D) wild-type and (E) ntltc41 embryos, note that expression in the notochord is not detectable. (G) In heterozygous doctt258/+ embryos, Ntl is not detectable in a few nuclei of the notochord (arrowhead in the enlargement). In (J) doctt258 embryos notochord expression of shh (blue) is only detectable in notochord cells which also express Ntl (brown, compare to Ntl expression in H). In regions to the left of the arrowhead (anterior), shh expression is restricted to the floor plate.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
shha WT standard conditions 10-13 somites notochord ISH
dharmath211/th211 standard conditions 10-13 somites notochord ISH
tbxta Ab1-ta WT standard conditions Bud axial mesoderm IHC
Ab1-ta Bud tail bud IHC
Ab1-ta 10-13 somites notochord IHC
Ab1-ta 10-13 somites tail bud IHC
Ab1-ta dharmath211/th211 standard conditions Bud axial mesoderm IHC
Ab1-ta Bud tail bud IHC
Ab1-ta doctt258/+ standard conditions 10-13 somites notochord IHC
Ab1-ta 10-13 somites tail bud IHC
Ab1-ta doctt258/tt258 standard conditions 10-13 somites notochord posterior region IHC
Ab1-ta 10-13 somites tail bud IHC
Ab1-ta nototk241/tk241 standard conditions Bud tail bud IHC
Ab1-ta tbxtatc41/tc41 standard conditions Bud Not Detected axial mesoderm IHC
twist2 WT standard conditions Bud axial mesoderm ISH
tbxtatc41/tc41 standard conditions Bud Not Detected axial mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.