FIGURE

Fig. 4

ID

ZDB-FIG-120224-7

Publication

Hugo et al., 2012 - A monocarboxylate transporter required for hepatocyte secretion of ketone bodies during fasting

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Fig. 4

Slc16a6a is a β-hydroxybutyrate transporter. (A) RT?PCR of mature slc16a6a and rpp0 transcripts at the indicated ages. (B) Whole-mount in situ hybridization with slc16a6a riboprobes. (C) The topology of the predicted wild-type and hypothetical rmn Slc16a6a proteins. (D) Ponceau S staining of nitrocellulose membranes following transfer of proteins resolved by sodium dodecyl sulfate polyacrylamide electrophoresis. (E) Immunoblotting with custom anti-Slc16a6a IgGs of the membrane in D. The membrane was deliberately overexposed in order to increase the chance of detecting a smaller protein band in the rmn mutant. (F) Current tracings of Xenopus oocytes expressing Slc16a6a (or control-injected oocytes, in black) that were perfused with monocarboxylic acids (5 mM) for the indicated time period. Oocytes were voltage-clamped to a holding potential of 70 mV. (G) Current?voltage relation of a Slc16a6a-expressing oocyte perfused with β-hydroxybutyrate.

Expression Data

Gene:	slc16a6a
Antibody:	Ab1-slc16a6a
Fish:	WIK slc16a6a^s951/s951; gz2Tg; gz4Tg; m1018Tg (WIK)
Anatomical Terms:	head intestinal bulb liver pronephros swim bladder whole organism (all 6)
Stage Range:	1-cell to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	slc16a6a^s951/s951; gz2Tg; gz4Tg; m1018Tg (WIK)
Observed In:	monocarboxylic acid transmembrane transporter activity
Stage:	Adult

Phenotype Detail

Acknowledgments

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