Fig. 5
- ID
- ZDB-FIG-120216-19
- Publication
- Mishima et al., 2012 - Translational inhibition by deadenylation-independent mechanisms is central to microRNA-mediated silencing in zebrafish
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miR-1 represses target mRNA in a deadenylation- and PABP-independent manner in zebrafish embryos. (A) Fluc reporter mRNA containing zebrafish pdlim1 32UTR. Red boxes indicate the target site for miR-1. (B) Scheme of the miR-1 repression assay in zebrafish embryos. (C) The poly(A) tail analysis of the Fluc-pdlim1 32UTR reporter mRNAs at six hours in the absence (-) or presence (+) of the miR-1 duplex. The left panel shows the reporter mRNA with a normal poly(A) tail [Fluc-pdlim1-poly(A)]. The right panel shows the reporter mRNA with the A98C10 tail (Fluc-pdlim1-A98C10). (D and E) The results of the miR-1 repression assay with reporter mRNA containing a normal poly(A) tail in the presence of control Myc-GFP. (F and G) Results of the miR-1 repression assay with reporter mRNA containing the A98C10 tail in the presence of control Myc-GFP. (H and I) Results of the miR-1 repression assay with reporter mRNA containing the A98C10 tail in the presence of Myc-Paip2. D, F, and H show normalized Fluc activity. E, G, and I show normalized Fluc mRNA levels, which were measured using qRT-PCR. The values of the experiments using miR-124 were set to one at each time point. The data shows averages of three independent experiments. Error bars show SD. |
| Gene: | |
|---|---|
| Fish: | |
| Anatomical Term: | |
| Stage: | Prim-5 |
| Fish: | |
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| Knockdown Reagent: | |
| Observed In: | |
| Stage: | Prim-5 |