ZFIN ID: ZDB-FIG-120131-24
Caron et al., 2012 - Wnt/β-catenin signaling directly regulates Foxj1 expression and ciliogenesis in zebrafish Kupffer’s vesicle. Development (Cambridge, England)   139(3):514-24 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Condition:
Anatomical Terms:
Stage Range: 10-13 somites to 26+ somites
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: 26+ somites to Long-pec

Fig. 6 Dkk1 induction impairs ciliogenesis in PD and OV. (A-D) Induction of Dkk1 downregulates foxj1 expression in PDs and OVs. foxj1a level in PDs was downregulated in transgenic Dkk1 embryos (B; 8/8) versus non-transgenic siblings (A). foxj1a expression in FP was not affected (B). foxj1b expression in the otic placode was downregulated in transgenic Dkk1 embryos (D; 8/8) versus non-transgenic siblings (C). Arrow indicates PDs, asterisk FP, and arrowhead the otic placode. (E,F) Induction of Dkk1 suppressed dnah9 expression in PDs and OVs (F; 11/11) compared with controls (E). White arrow represents PDs, and white arrowhead the otic placode. Shown are dorsal views (A,B) and lateral views (C-F) of 10-somite staged embryos with anterior to the left. (G-J) Induction of Dkk1 results in shorter and fewer cilia in PDs and OVs. Cilia were visualized by anti-tubulin antibody staining of 26-somite staged embryos. White arrow indicates tethering cilia in OVs. (K,L) Quantification of cilia length in PDs (K) and OVs (tethering cilia) (L). Data are represented as mean±s.d. Approximately 9-18 embryos were used for each group. *P<0.01. (M-O) Induction of Dkk1 results in kidney cysts and otolith malformation. Cystic distension of PD (arrow in M; 45/73) was observed at 24 hpf. One otolith (arrowhead in O; 89/95) was seen at 2 dpf. Tg(hsp:dkk1-GFP) embryos were heat activated at 60% epiboly.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
foxj1a zf418Tg control 10-13 somites floor plate ISH
10-13 somites pronephric duct ISH
zf418Tg heat shock 10-13 somites floor plate ISH
10-13 somites pronephric duct ISH
foxj1b zf418Tg control 10-13 somites otic placode ISH
zf418Tg heat shock 10-13 somites otic placode ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tuba IHC zf418Tg control 26+ somites otic vesicle motile cilium
IHC 26+ somites pronephric duct motile cilium
IHC zf418Tg heat shock 26+ somites otic vesicle motile cilium
IHC 26+ somites pronephric duct motile cilium
Phenotype Details
Fish Conditions Stage Phenotype
zf418Tg heat shock 26+ somites otic vesicle motile cilium decreased amount, abnormal
26+ somites otic vesicle motile cilium decreased length, abnormal
26+ somites pronephric duct motile cilium decreased amount, abnormal
26+ somites pronephric duct motile cilium decreased length, abnormal
Prim-5 pronephros cystic, abnormal
Long-pec otolith decreased amount, abnormal
Long-pec otolith malformed, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development