FIGURE

Fig. 4

ID

ZDB-FIG-120125-7

Publication

Leacock et al., 2012 - A zebrafish transgenic model of Ewing's sarcoma reveals conserved mediators of EWS-FLI1 tumorigenesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

EWS-FLI1 induces developmental defects in zebrafish embryos. (A) Non-transgenic (GFP–) embryos are wild type. (B) Transgenic embryos (GFP+) carrying a heat-shock-inducible EWS-FLI1 transgene exhibit pericardial edema (arrow) and reduced head and eye size. (C) A morpholino against the transgene decreases the penetrance of the pericardial edema phenotype. (D) Percentage of transgenic embryos exhibiting pericardial edema is decreased using a morpholino targeting the EWS-FLI1 transgene. (E) RT-PCR for EWS-FLI1 shows low level of expression without heat shock (–HS) and increased expression following heat shock (+HS). no RT, no RT negative control.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sw3Tg
Observed In:	eye head pericardial cavity
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.