ZFIN ID: ZDB-PUB-111025-2
A zebrafish transgenic model of Ewing's sarcoma reveals conserved mediators of EWS-FLI1 tumorigenesis
Leacock, S.W., Basse, A.N., Chandler, G.L., Kirk, A.M., Rakheja, D., and Amatruda, J.F.
Date: 2012
Source: Disease models & mechanisms   5(1): 95-106 (Journal)
Registered Authors: Amatruda, James F., Basse, Audrey, Kirk, Anne, Leacock, Stefanie
Keywords: none
Microarrays: GEO:GSE31185, GEO:GSE31186, GEO:GSE31217
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Cell Transformation, Neoplastic/genetics
  • Cell Transformation, Neoplastic/pathology*
  • Disease Models, Animal
  • Embryo, Nonmammalian/metabolism
  • Embryo, Nonmammalian/pathology
  • Embryonic Development/genetics
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Neoplasm Transplantation
  • Oncogene Proteins, Fusion/genetics
  • Oncogene Proteins, Fusion/metabolism*
  • Proto-Oncogene Protein c-fli-1/genetics
  • Proto-Oncogene Protein c-fli-1/metabolism*
  • RNA-Binding Protein EWS/genetics
  • RNA-Binding Protein EWS/metabolism*
  • Sarcoma, Ewing/genetics
  • Sarcoma, Ewing/pathology*
  • Transgenes/genetics
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism*
PubMed: 21979944 Full text @ Dis. Model. Mech.
FIGURES
ABSTRACT

Ewing’s sarcoma, a malignant bone tumor of children and young adults, is a member of the small-round-blue-cell tumor family. Ewing’s sarcoma family tumors (ESFTs), which include peripheral primitive neuroectodermal tumors (PNETs), are characterized by chromosomal translocations that generate fusions between the EWS gene and ETS-family transcription factors, most commonly FLI1. The EWS-FLI1 fusion oncoprotein represents an attractive therapeutic target for treatment of Ewing’s sarcoma. The cell of origin of ESFT and the molecular mechanisms by which EWS-FLI1 mediates tumorigenesis remain unknown, and few animal models of Ewing’s sarcoma exist. Here, we report the use of zebrafish as a vertebrate model of EWS-FLI1 function and tumorigenesis. Mosaic expression of the human EWS-FLI1 fusion protein in zebrafish caused the development of tumors with histology strongly resembling that of human Ewing’s sarcoma. The incidence of tumors increased in a p53 mutant background, suggesting that the p53 pathway suppresses EWS-FLI1-driven tumorigenesis. Gene expression profiling of the zebrafish tumors defined a set of genes that might be regulated by EWS-FLI1, including the zebrafish ortholog of a crucial EWS-FLI1 target gene in humans. Stable zebrafish transgenic lines expressing EWS-FLI1 under the control of the heat-shock promoter exhibit altered embryonic development and defective convergence and extension, suggesting that EWS-FLI1 interacts with conserved developmental pathways. These results indicate that functional targets of EWS-FLI1 that mediate tumorigenesis are conserved from zebrafish to human and provide a novel context in which to study the function of this fusion oncogene.

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