FIGURE

Fig. 6

ID

ZDB-FIG-111215-21

Publication

Nguyen et al., 2011 - A high level of liver-specific expression of oncogenic Kras^V12 drives robust liver tumorigenesis in transgenic zebrafish

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Fig. 6

Kras^V12-induced p53-dependent senescence in the pre-neoplastic liver. (A) Oncogenic Kras^V12-induced senescence at an early stage of liver tumor development. SA-βgal staining was performed on liver cryosections from 3- and 9-mpf kras^V12 transgenic and WT fish (tp53^+/+). (B) Western blots of total proteins from liver hyperplasia (H), carcinoma (C) and age-matched WT liver to evaluate the levels of tumor suppressor p53 together with its target, p21 Waf1/Cip1, and its regulator, phospho-MDM2, during tumorigenesis. β-actin, internal control for equal loading. (C) Acceleration of liver tumor onset in homozygous tp53^M214K mutant transgenic fish. Percentages of tumors observed from kras^V12 transgenic fish with different p53 backgrounds at different time points are shown. (D) Suppression of senescence induced by oncogenic kras^V12 in tp53^-/- background. SA-βgal staining was performed with four biological replicates in each group. Scale bars: 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tp53^zdf1/+; gz15Tg tp53^zdf1/zdf1; gz21Tg
Observed In:	liver liver hepatocellular carcinoma oncogene-induced cell senescence
Stage:	Adult

Phenotype Detail

Acknowledgments

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