Fig. 7

swap70 loss-of-function does not dramatically alter spinal cord development. All images show transverse sections through trunk spinal cord with dorsal up. (A, B) Anti-PrkCi immunocytochemistry performed on control and MO^swap70-sp-injected larva at 3 dpf. PrkCi labeling of apical membrane (arrows) and EGFP labeling of olig2⁺ radial glia (arrowheads) is indistinguishable between control and experimental animals whereas fewer EGFP⁺ OPCs (asterisks) are evident following MO^swap70-sp injection. (C, D) Anti-Zrf1 immunocytochemistry to label radial glia at 3 dpf. The shape and number of Zrf1⁺ radial fibers look similar in wild type and MO^swap70-sp-injected larva. Arrowheads and asterisks mark EGFP⁺ radial glia and OPCs, respectively. (E, F) sox2 mRNA expression in the spinal cord at 3 dpf. More medial spinal cord cells express sox2 in MO^swap70-sp-injected larva. (G–I) Anti-pH3 immunocytochemistry to label M-phase cells (red) on spinal cord sections of 54 hpf Tg(olig2:EGFP) embryos. In MO-injected embryos more spinal cord cells are positive for pH3. (J) Quantification of pH3 labeling. Y axis indicates total pH3 positive cells on 15 sections per embryo. N indicates number of embryos analyzed. (K) Quantification of BrdU incorporation to label S-phase cells. Embryos were soaked in BrdU solution from 52 hpf to 62 hpf. Y axis indicates number of BrdU positive cells number per section.