ZFIN ID: ZDB-FIG-111128-59
Zuniga et al., 2011 - Gremlin 2 regulates distinct roles of BMP and Endothelin 1 signaling in dorsoventral patterning of the facial skeleton. Development (Cambridge, England)   138(23):5147-5156 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Prim-25
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 1 Facial skeletal defects upon Bmp4 or Edn1 misexpression. (A-F) Confocal projections of in situ hybridization for dlx2a (blue), bmp4 (green) and edn1 (red) at 24 hpf (A-C) and 36 hpf (D-F) in wild type. Mandibular (1) and hyoid (2) arches are labeled, as well as dorsal (D), intermediate (I) and ventral (V) domains. (G-I) Ventral views (top) and schematics (below) of 5 dpf facial skeletons in control hsp70I:Gal4 (G) and hsp70I:Gal4; UAS:Bmp4 larvae subjected to a 4-hour heat-shock (H), and hsp70I:Gal4; UAS:Edn1 larvae subjected to an 8-hour heat-shock (I). Cartilage is blue and bone red. Schematics show dorsal (green), intermediate (red) and ventral (blue) regions with dermal bones lightly shaded. Hm (black arrow), Pq (blue arrow) and Ptp (grey arrow) were transformed (arrowheads) in UAS:Bmp4 and UAS:Edn1 larvae. In the intermediate second arch, the joint (asterisk) and symplectic bone were lost in UAS:Bmp4 but not UAS:Edn1 larvae. M, Meckel’s cartilage; Pq, palatoquadrate cartilage; Ptp, pterygoid process; Sy, symplectic cartilage; Hm, hyomandibular cartilage; Ch, ceratohyal cartilage; Op, opercular bone; Br, branchiostegal ray bone. Scale bars: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
bmp4 WT standard conditions Prim-5 mandibular arch skeleton ectoderm ISH
Prim-5 ventral hyoid arch ectoderm ISH
edn1 WT standard conditions Prim-5 ventral hyoid arch ectoderm ISH
Prim-25 ventral hyoid arch ectoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
el249Tg; kca4Tg standard conditions Day 5 hyosymplectic cartilage malformed, abnormal
Day 5 palatoquadrate arch malformed, abnormal
Day 5 palatoquadrate arch morphology, abnormal
Day 5 pharyngeal arch 1 malformed, abnormal
Day 5 symplectic normal amount, normal
el49Tg; kca4Tg standard conditions Day 5 hyosymplectic cartilage malformed, abnormal
Day 5 palatoquadrate arch malformed, abnormal
Day 5 palatoquadrate arch morphology, abnormal
Day 5 pharyngeal arch 1 malformed, abnormal
Day 5 symplectic absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development