ZFIN ID: ZDB-FIG-111102-4
Gutnick et al., 2011 - The hypothalamic neuropeptide oxytocin is required for formation of the neurovascular interface of the pituitary. Developmental Cell   21(4):642-654 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-25 to Protruding-mouth

Fig. 4

Morphogenesis of the Neurohypophysis

(A–C) Dynamics of hypophyseal innervation. Images of transgenic otpb:caax-EGFP embryos reveal that innervation of the hypophysis by neurons of the neurosecretory preoptic nucleus (NPO) occurs as early as 36 hr postfertilization and becomes well established by 54 hr. Scale bar, 20 μm.

(D–F) Dynamics of neurovascular interface. Images of double transgenic otpb:caax-EGFP;fli1:dsRed embryos at different developmental stages showing innervation of the neurohypophysis prior to hypophyseal vessel formation.

(G) Dynamics of hypophyseal vascularization. Time-lapsed (48–72 hr) confocal microscopy was used to track vascular endothelial cells in the ventral diencephalon of vegfr2:Cherry embryos. The upper panels display snapshots of critical stages in the formation of the hypophyseal loop-like structure. A schematic representation of the corresponding images with arterial (red) and venous (blue) color-coding is shown at the bottom.

CrDI, cranial division of the internal carotid artery; HyA, hypophyseal artery; HyV, hypophyseal veins; OA, optic artery; PHS, primary head sinus; PLA, palatocerebral artery. The asterisk (*) demarcates the location of the neurohypophysis. See also Movie S6.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRedx um13Tg; zc49Tg standard conditions Prim-25 hypophysis vascular endothelium IFL
Long-pec hypophysis vascular endothelium IFL
Protruding-mouth hypophysis vascular endothelium IFL
EGFP um13Tg; zc49Tg standard conditions Prim-25 neurohypophysis axon terminus IFL
Prim-25 neurosecretory neuron axon IFL
Prim-25 neurosecretory neuron axon terminus IFL
Prim-25 neurosecretory neuron neuronal cell body IFL
Prim-25 preoptic area neurosecretory neuron IFL
Long-pec neurohypophysis axon terminus IFL
Long-pec neurosecretory neuron axon IFL
Long-pec neurosecretory neuron axon terminus IFL
Long-pec neurosecretory neuron neuronal cell body IFL
Long-pec preoptic area neurosecretory neuron IFL
Protruding-mouth neurohypophysis axon terminus IFL
Protruding-mouth neurosecretory neuron axon IFL
Protruding-mouth neurosecretory neuron axon terminus IFL
Protruding-mouth neurosecretory neuron neuronal cell body IFL
Protruding-mouth preoptic area neurosecretory neuron IFL
zc49Tg standard conditions Prim-25 neurohypophysis axon terminus IFL
Prim-25 neurosecretory neuron axon IFL
Prim-25 neurosecretory neuron axon terminus IFL
Prim-25 neurosecretory neuron neuronal cell body IFL
Prim-25 preoptic area neurosecretory neuron IFL
Long-pec neurohypophysis axon terminus IFL
Long-pec neurosecretory neuron axon IFL
Long-pec neurosecretory neuron axon terminus IFL
Long-pec neurosecretory neuron neuronal cell body IFL
Long-pec preoptic area neurosecretory neuron IFL
Protruding-mouth neurohypophysis axon terminus IFL
Protruding-mouth neurosecretory neuron axon IFL
Protruding-mouth neurosecretory neuron axon terminus IFL
Protruding-mouth neurosecretory neuron neuronal cell body IFL
Protruding-mouth preoptic area neurosecretory neuron IFL
mCherry s896Tg standard conditions Long-pec hypophysis vascular endothelium IHC
Pec-fin hypophysis vascular endothelium IHC
Protruding-mouth hypophysis vascular endothelium IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 21(4), Gutnick, A., Blechman, J., Kaslin, J., Herwig, L., Belting, H.G., Affolter, M., Bonkowsky, J.L., and Levkowitz, G., The hypothalamic neuropeptide oxytocin is required for formation of the neurovascular interface of the pituitary, 642-654, Copyright (2011) with permission from Elsevier. Full text @ Dev. Cell