ZFIN ID: ZDB-FIG-111102-3
Gutnick et al., 2011 - The hypothalamic neuropeptide oxytocin is required for formation of the neurovascular interface of the pituitary. Developmental Cell   21(4):642-654 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Long-pec

Fig. 3

3D Structure of the Hypophyseal Neurovascular Interface

(A and B) Identification of hypophyseal arteries and veins by real-time analysis of hypophyseal blood flow.

(A) Confocal time-lapsed images (t1–t10) of a single gata1+ blood cell (arrowhead) traveling from the hypophyseal artery into the neurohypophysis and its outflow through the hypophyseal veins. Superposition of all time points and the direction of blood flow (red arrow) are shown in the right panel. Adjacent Prl-RFP+ adenohypophyseal cells (arrow) served as a positional landmark.

(B) Schematic map of ventral head vasculature of the zebrafish larvae, including the newly annotated hypophyseal artery (red) and veins (blue). AH, adenohypophysis, CrDI, cranial division of the internal carotid artery; HyA, hypophyseal artery; HyV, hypophyseal veins; OA, optic artery; PLA, palatocerebral artery.

(C) Schematic representation of the 3D structure of the zebrafish pituitary depicting the various cellular components of the adeno- and neurohypophysis. A longitudinal section of this structure is shown on the right.

See also Figure S2 and Movie S5.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed s843Tg; sd2Tg; zf113Tg standard conditions Long-pec hypophysis nucleate erythrocyte IFL
EGFP s843Tg; sd2Tg; zf113Tg standard conditions Long-pec hypophysis vascular endothelium IFL
RFP s843Tg; sd2Tg; zf113Tg standard conditions Long-pec adenohypophysis IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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Reprinted from Developmental Cell, 21(4), Gutnick, A., Blechman, J., Kaslin, J., Herwig, L., Belting, H.G., Affolter, M., Bonkowsky, J.L., and Levkowitz, G., The hypothalamic neuropeptide oxytocin is required for formation of the neurovascular interface of the pituitary, 642-654, Copyright (2011) with permission from Elsevier. Full text @ Dev. Cell